TESTRÖ-accessories
TESTRÖ-QUANT 3 S (TQ 3 S)
TESTRÖ-Thermocult
Nitrite/Nitrate-sticks
PH-Wert-Streifen
TQ 3S serves for quantifying bacteria, moulds and yeasts, and hygienically relevant bacteria, in water soluble cooling lubricants and other liquids.
1. Characterisation
TESTRÖ-Quant 3S is a threepart medium that is attached to a lid. This lid seals a little tube, in which the mediums are protected against dehydration and contamination.
1. Medium for determining the total bacteria count. 2. Pink medium for coliform bacteria. 3. Medium for moulds and yeasts. |
On medium 1 colonies of predominantly bacteria cultivate after 2-3 days, on medium 2 colonies of yeasts and moulds only. On the medium for hygienically relevant bacteria only red coloured colonies are to be analysed. The cultivation of moulds and yeasts and colourless colonies of bacteria on the pink medium is possible. The analysis, however, is not affected by them.
2. Implementaion of the meduim analysis
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1000/ml
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10 000/ml
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100 000/ml
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1 000 000/ml
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Illustration 1: Comparison pictures showing bacterial infestation
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Only the amount of the single colonies but not their size is relevant for the analysis. In case of a very high colony count a bacterial carpet has been cultivated, i.e. between the colonies there are no spaces any more. The whole surface is then abundantly covered and therefore shines. In this case the sample can be watered down for determining the colony count (see 2.8). Universal limiting values that justify the application of preservatives cannot be denounced but ensue by experience.
3. Analysis
For reading of the result the TQ 3S can be taken from the receptacle, if this should have got steamed up. Bacteria and fungi do not have legs and therefore do not jump away from the medium. You must not touch the colonies, in any case unnecessary.
3.1 Total bacteria (see medium 1) The determination of the total bacteria ensues with the aid of the comparison pictures, i.e. the colonies are not counted. 3.2 Coliform bacteria (medium 2) The pink-coloured part of the divided parts shows proof of coliform bacteria. Coliform bacteria cultivate strikingly red coloured colonies and are therefore well distinguishable from other colourless colonies that also cultivate on this side. 3.3 Fungi (medium 3) 3.3.1 Moulds 3.3.2 Yeasts |
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Disposal of used culture mediums
On the incubated mediums are cultures of microorganisms. They must not be touched. After analysing them, the mediums have to be destroyed.The killing of the microorganisms can ensue by burning the receptacles together with the mediums depositing or by depositing the mediums and receptacles into an aggressive disinfectant. In the latter case the receptacles must be unscrewed and both the parts deposited into the disinfectant over night.
Storage
TESTRÖ-Quant 3S that have not been opened can be preserved for up to 6 months when stored in a cool and dark place. When storing the TQ 3S in the refrigerator they can be preserved for approximately one month longer (no guarantee).
The ideal incubator for small-scale bacteriology, e.g. in emulsions and solutions
Technical data:
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TESTRÖ-Thermocult - incubator for small-scale bacteriology
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TESTRÖ-Thermocult
Multifunctional |
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Easy |
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Reliable |
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Nitrite/Nitrate-Test sticks (100 pcs.)
for semiquantitative determination of nitrite and nitrate (1-80 mg per lit. NO2-), (10-500mg per lit. NO3-)